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Titre

Microscopy and Imaging Course: course 14B044 Electron Microscopy

Dates

23.04.2020-28.05.2020

Lang EN Workshop language is English
Organisateur(s)

Dr Christoph Bauer, UNIGE

Intervenant(s)

Christoph Bauer : CB
Yashar Sadian : YS
Sylvain Loubéry : SL
Rossana Martini : RM
Agatha Martignier : AM
Jérôme Bosset : JB
Laurent Brodier : LB
Christel Genoud : CG
Lena Hyvärinen : LH

all from UNIGE

Description

This course will cover theory and practice of electron microscopy.

Programme

Teachers and Speakers

Christoph Bauer : CB

Yashar Sadian : YS

Sylvain Loubéry : SL

Rossana Martini : RM

Agatha Martignier : AM

Jérôme Bosset : JB

Laurent Brodier : LB

Christel Genoud : CG

Lena Hyvärinen : LH

 

 

Lecture title and subject

 
 

Teacher (s)

 
 

Possible date

 
 

Schedule

 
 

Introduction: Teachers and students present themselves , students declare motivation. CB explains organization of course and exam modalities

 
 

CB,YS,SL,RM,AM,JB

 
 

Thursday, 23.04. AM

 
 

10 :15- 11 :00

 
 

TEM (transmission electron microscopy): introduction to transmitted electron microscopy: history, definitions, and physical basics

 
 

CB

 
 

Thursday, 23.04. PM

 
 

14 :00- 15 :00

 
 

Chemicals used in electron microscopy, their function and safety aspects for their use

 
 

SL

 
 

Friday, 24.04. AM

 
 

11:00 – 11:45

 
 

TEM elements, built and setup of instrument

 
 

CB

 
 

Friday, 24. 04 PM

 
 

14 :00- 15 :00

 
 

Specimen preparation I : general aspects of fixation, dehydration and resin embedding

 
 

SL

 
 

Wednesd., 29.04. AM

 
 

10:15 – 11:30

 
 

Specimen preparation II sample challenges: skin, cell monolayers, chicken tissue

 
 

CB and LB

 
 

Wednesd., 29.04. PM

 
 

 

 
 

Specimen preparation II: High pressure freezing and freeze substitution with virtual live demo

 
 

CB

 
 

Thursd. 30.04. AM

 
 

 

 
 

Grids, glow discharge and negative staining protocols

 
 

YS

 
 

Thursd. 30.04. PM

 
 

 

 
 

Microtome use and sectioning of resin embedded tissues and cells

 
 

CB

 
 

Thursday, 07.05. AM

 
 

 

 
 

Live virtual demo of microtome use and sectioning with glass knives and diamond

 
 

CB

 
 

Thursday, 07.05 PM

 
 

 

 
 

Scanning electron microscopy

 
 

RM

 
 

Friday, 08.05 AM

 
 

9:15 to 11:00

 
 

Live virtual SEM demo

 
 

AM and JB

 
 

Friday. 08.05 PM

 
 

13:00 – 14:00 JB

14:15 – 15:15 AM

 
 

“3D electron microscopy”

 
 

CG

 
 

Thursday, 14.05 AM

 
 

 

 
 

Cryo-TEM: specimen preparation and cryo-imaging

 
 

YS

 
 

Thursday, 14.05. PM

 
 

 

 
Lieu

Via ZOOM session, due to Coronavirus situation

Crédits

4

Information
Places

12

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