Lecture title and subject

Teacher (s)

Possible date

Schedule

Introduction: Teachers and students present themselves , students declare motivation. CB explains organization of course and exam modalities

CB,YS,SL,RM,AM,JB

Thursday, 23.04. AM

10 :15- 11 :00

TEM (transmission electron microscopy): introduction to transmitted electron microscopy: history, definitions, and physical basics

CB

Thursday, 23.04. PM

14 :00- 15 :00

Chemicals used in electron microscopy, their function and safety aspects for their use

SL

Friday, 24.04. AM

11:00 – 11:45

TEM elements, built and setup of instrument

CB

Friday, 24. 04 PM

14 :00- 15 :00

Specimen preparation I : general aspects of fixation, dehydration and resin embedding

SL

Wednesd., 29.04. AM

10:15 – 11:30

Specimen preparation II sample challenges: skin, cell monolayers, chicken tissue

CB and LB

Wednesd., 29.04. PM

Specimen preparation II: High pressure freezing and freeze substitution with virtual live demo

CB

Thursd. 30.04. AM

Grids, glow discharge and negative staining protocols

YS

Thursd. 30.04. PM

Microtome use and sectioning of resin embedded tissues and cells

CB

Thursday, 07.05. AM

Live virtual demo of microtome use and sectioning with glass knives and diamond

CB

Thursday, 07.05 PM

Scanning electron microscopy

RM

Friday, 08.05 AM

9:15 to 11:00

Live virtual SEM demo

AM and JB

Friday. 08.05 PM

13:00 – 14:00 JB

14:15 – 15:15 AM

“3D electron microscopy”

CG

Thursday, 14.05 AM

Cryo-TEM: specimen preparation and cryo-imaging

YS

Thursday, 14.05. PM