Introduction to Plant Metabolomics

02 and 03 June 2022

Interuniversity Doctoral Program in Organismal Biology

Please contact the doctoral program coordinator Dr Emilie Genty ([email protected])

Dr Pierre-Marie Allard, University of Fribourg

Dr Gaétan Glauser, MS and metabolomics unit, Neuchâtel platform of Analytical Chemistry (NPAC), UNINE

Dr Emmanuel Defossez,

This practical short course intends to provide an overview of the different steps needed to perform mass spectrometry-based plant metabolomics. The course will take place in the laboratory and in computer rooms where participants will have the opportunity to become familiar with sample preparation, analysis by HPLC-MS, data processing, and compound identification.

Preliminary Program

DAY 1

*** Morning - 8h45-12h15 (botanical garden, address TBA)

Introduction to plant Metabolomics
- Definitions
- Sample preparation for Metabolomics
- Analytical techniques for Metabolomics
- Data processing and metabolite annotation

Collection of plant samples at the botanical garden
- Plant selection
- Plant collection and storage

*** Afternoon - 13h45-16h45 (UniMail, Building G, lab A28)

Laboratory session
- Sample preparation of collected samples
- Anaylsis by HPLC-MS/MS
- Visualization of raw data

 

DAY 2

*** Morning - 9h00-12h00 (UniMail, Building X, room XX)

Data processing
- Overview of the workflow
- Data conversion
- Data pre-treatment (peak picking, deconvolution, alignment)

*** Afternoon - 13h30-16h30 (UniMail, Building X, room XX)

Bioinformatics
- Biostatistics
- Molecular Networks
- Metabolite annotation (MSFinder, GNPS, Sirius)

University of Neuchâtel, Faculté des Sciences, Rue Emile-Argand 11

2

!! double registration: through the pharma CUSO website, and also here: biologie.cuso.ch

6